Journal: Cell reports

Article Title: Staphylococcus aureus exploits lipoic acid salvage to combat host oxidative stress

doi: 10.1016/j.celrep.2025.116095

Figure Lengend Snippet: (A) Bacterial recovery of the indicated strains from the kidneys and peritoneal cavity of Swiss Webster mice 24 h after intraperitoneal injection ( n = 24). * p < 0.05 and ***, p < 0.001, calculated by the Kruskal-Wallis test with Dunn’s multiple comparisons test. (B) Bacterial recovery of the indicated strains from the kidneys of C57BL/6 mice 24 h after bloodstream infection ( n = 16). * p < 0.05 and ** p < 0.01 by Kruskal-Wallis test with Dunn’s multiple comparisons test. (C) Bacterial recovery of the indicated strains from the peritoneal cavity of C57BL/6 and Cybb − mice 24 h after intraperitoneal injection with 1 × 10 8 CFUs ( n = 20) or 1 × 10 7 CFUs ( n = 10). ** p < 0.01, *** p < 0.001 by unpaired t test (1 × 10 8 CFUs) or Mann-Whitney test (1 × 10 7 CFUs). (D) Outgrowth of the indicated strains after infection with isolated macrophages from Swiss Webster mice. (E) Outgrowth of the indicated strains after infection with isolated macrophages from Swiss Webster mice that were treated with vehicle control (water) or the NADPH oxidase inhibitor gp91ds-tat (50 μM). (F) Outgrowth of the indicated strains after infection with isolated macrophages from C57BL/6 and Cybb − mice. ** p < 0.01, *** p < 0.001, **** p < 0.0001 by two-way ANOVA with Tukey’s post hoc test (D–F). Data are represented as individual values with the median indicated as a solid line.

Article Snippet: Mouse Swiss Webster serum , Innovative Research Inc , Cat # 50-203-5738.

Techniques: Injection, Infection, MANN-WHITNEY, Isolation, Control

Journal: Journal of Immunotoxicology

Article Title: An ELISA for detection of complement-bound circulating immune complexes in mice

doi: 10.1080/1547691x.2019.1599471

Figure Lengend Snippet: Figure 1. Preparation of the positive control. The positive control was used as quality control in the enzyme-linked immunosorbent assay (ELISA). Mouse serum con- taining mouse immunoglobulin (Ig) and inactivated complement was incubated with heat-aggregated human IgG (“Complement Activator”) to stimulate complement activation by the classical complement pathway. Monomeric mouse IgG was able to bind to aggregated human IgG, leading to formation of complement bound hIgG-mIgG complexes. HRP (horse radish peroxidase) signal.

Article Snippet: In brief, mouse serum complement (Innovative Research, Novi, MI, cat. no IMS-C57BL6-COMPL) was incubated at 37 C for 90min with 1:100 HAGG (Complement Activator, Quidel, San Diego, CA, cat. #A114), followed by inactivation by addition of ice-cold 500mM ethylenediamine-tetraacetic acid (EDTA; to final concentration of 20mM) and storage (undiluted) at 80 C until analysis.

Techniques: Positive Control, Control, Enzyme-linked Immunosorbent Assay, Incubation, Activation Assay

Journal: Journal of thrombosis and thrombolysis

Article Title: Metal ion chelation enhances tissue plasminogen activator (tPA)-induced thrombolysis: an in vitro and in vivo study.

doi: 10.1007/s11239-021-02600-6

Figure Lengend Snippet: Fig. 5 Photothrombosis and thrombolysis in vivo in the mouse femo- ral artery. A The femoral artery (shown by an arrow) of CD1 mouse. Scale bar: 1 cm. B(1–3) Photothrombotic occlusion of the femoral artery. B-1 Clot indicated by an arrow occluded the femoral artery downstream (outlined by dash lines) of the thrombus formation. The artery occlusion created a blood-free field. B-2. Reperfusion of the femoral artery by the treatment of tPA. B-3 Darker colored clot in a non-reperfused femoral artery. The image (B-3) was taken 30 min after establishing photothrombotic clot. Scale bar for B: 1 mm. C Change of color intensity of clot (thrombus) in non-reperfused femo- ral artery over time. Images of the thrombus were taken every 5 min and was analyzed by ImagePro software. Change of thrombus color intensity was expressed as percentage increase and calculated accord- ing to the formula (F -F0)/F0· %. Values are mean ± SEM. n = 7–9

Article Snippet: Citrated blood of male CD1 mice was purchased from Innovative Research (Novi, MI) and BioChemed Services (Winchester, VA).

Techniques: In Vivo, Software

Journal: EMBO Reports

Article Title: FAM69C functions as a kinase for eIF2α and promotes stress granule assembly

doi: 10.15252/embr.202255641

Figure Lengend Snippet: A Immunoblot analysis of phosphorylated eIF2α in BV2 cells. Primed (1 μg/ml LPS for 3 h) or unprimed BV2 cells were treated with mock, 5 mM ATP or 20 μM nigericin for 40 min, and lysates were immunoblotted with p‐eIF2α (S51) antibody. Representative blots, n = 3 biologically independent experiments. B Quantitative analysis of the phosphorylation level of eIF2α normalized with total eIF2α. ** P value < 0.01, paired two‐tailed t ‐test, n = 3 biologically independent experiments. Mean ± SD. For ATP versus mock, P value = 0.0047; For LPS + ATP versus LPS, P value = 0.0011.

Article Snippet: The BV2 microglial cell line was purchased from Procell Co., Ltd. and has been authenticated recently (#CL‐0493, Wuhan, China).

Techniques: Western Blot, Two Tailed Test